The rainbow trout GR (rtGR)
has been cloned by Ducouret
et al (1995). Because of its low homology to other known GR
sequences, and for the sake of visual clarity, we chose to compare
full length tGR to ratGR seperately from the GR from other species.
Only the DNA and hormone binding domains are shown in the cross
species comparison (the N-terminal domains are only 31% identical
even with the inclusion of 52 gaps).
This comparisson was obtained using a gapped Blast search without filtering, run on 12/17/97.
sp|P49843|GCR_ONCMY GLUCOCORTICOID RECEPTOR (GR) Length = 758
>gi|2133961|pir||S60586 glucocorticoid receptor - rainbow
trout
>gi|995677 (Z54210) glucocorticoid receptor [Oncorhynchus
mykiss]
sp|P06536|GCR_RAT GLUCOCORTICOID RECEPTOR (GR) Length = 795
Score = 654 bits (1669), Expect = 0.0
Identities = 366/713 (51%), Positives = 478/713 (66%), Gaps = 64/713
(8%)
Rat: 108 GLYMGETETKVMGNDLGY--PQQGQLGLSSGETDFRLLEESIANLNRSTSVPENPKSSTS 165
GL + E E KVM + PQ+ Q E +F LLE SIA+LNRS + P SS
Trout: 75 GLTIEEAEVKVMEKAIRMQQPQKPQQNQQLFE-NFALLEASIADLNRSNT----PGSSV- 128
Rat: 166 ATGCATPTEKEFPKTHSDASSEQQNRKSQTGTNGGSVKLYPTDQ-----STFDLLKDLEF 220
P + KT + + ++ + + G S K ++ +T D+L+DL+
Trout: 129 ---LGRPHDLFSLKTENFSPMDKDRLDMGSVSFGQSQKDLDVNERLLGDNTMDILQDLDL 185
Rat: 221 SAGSPSKD----TNESPWRSDLLIDENLLSPLAGEDDPFLLEGNTNEDCKPLILPDTKPK 276
+ ++E+ + S L +++ LL E P ++ + +C + D + +
Trout: 186 PGSLSDLNEFYVSDEAAFLSSLSVEDVLLEDGNMETKP--IDCSNGGNCTNVDSADQQKQ 243
Rat: 277 IKDTGDTILSSPSSVALPQVKTEKD---DFIELCTPGVIKQEKLGPVYCQASFSGTNIIG 333
+ + G V++P +KTE+D FI+LCTPGVIKQE +CQ S
Trout: 244 LLEAG---------VSMPVIKTEEDADTSFIQLCTPGVIKQENDRRSFCQISSLDLPSTH 294
Rat: 334 NKMSAISVHGVSTSGGQMYHYDMNTAS-LSQQQDQKPVFNVIPPIPVGSENWNRCQGSGE 392
N +IS G Y Y NT++ +S QQDQKPVF + PP+P S++WNR G+G
Trout: 295 NSAGSIS--------GPSYPYGANTSTAVSLQQDQKPVFGLYPPLPSVSDSWNR--GNGY 344
Rat: 393 DSLTSLGALNFPGRSVFSNGYSSPGMRPDVSSPPSSSSAA-TGPPPKLCLVCSDEASGCH 451
+ + + + +FP G+SSP RP+ S SS+ A +GP K+CLVCSDEASGCH
Trout: 345 ATGSGMSSSSFPV------GFSSPKARPEASGSASSAPAKPSGPTHKICLVCSDEASGCH 398
Rat: 452 YGVLTCGSCKVFFKRAVEG---------QHNYLCAGRNDCIIDKIRRKNCPACRYRKCLQ 502
YGVLTCGSCKVFFKRAVEG QHNYLCAGRNDCIIDKIRRKNCPACR+RKCLQ
Trout: 399 YGVLTCGSCKVFFKRAVEGWRARQNTDGQHNYLCAGRNDCIIDKIRRKNCPACRFRKCLQ 458
Rat: 503 AGMNLEARKTKK--KIKGIQQATAGVSQDTSENPNKTIVPAALPQLTPTLVSLLEVIEPE 560
AGMNLEARK KK ++KG QQ T + + ++P ++PQL PT++SLL+ IEPE
Trout: 459 AGMNLEARKNKKLIRLKG-QQTTMEPNPPPPDERACALIPKSMPQLVPTMLSLLKAIEPE 517
Rat: 561 VLYAGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAILGLRNLHLDDQMTLLQYSWMF 620
+Y+GYDS++PD++ R+MTTLN LGG+QV++AVKWAK++ G RNLHLDDQMTLLQ SW+F
Trout: 518 AIYSGYDSTIPDTSTRLMTTLNRLGGQQVVSAVKWAKSLPGFRNLHLDDQMTLLQCSWLF 577
Rat: 621 LMAFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFVSSELQRLQVSYEE 680
LM+F LGWRSY+Q +G +LCFAPDL+IN++RM LP M DQC+ ML +S+E RLQVSY+E
Trout: 578 LMSFGLGWRSYQQCNGGMLCFAPDLVINDERMKLPYMTDQCEQMLKISTEFVRLQVSYDE 637
Rat: 681 YLCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLL 740
YLCMK LLLLS+VPK+GLKSQ +FDEIRMTYIKELGKAIVKRE NSSQNWQRFYQLTKLL
Trout: 638 YLCMKVLLLLSTVPKDGLKSQAVFDEIRMTYIKELGKAIVKREENSSQNWQRFYQLTKLL 697
Rat: 741 DSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFH 793
DSM E+V LL CF TF++K++S+EFPEMLAEII+NQ+PK+ +G++K LLFH
Trout: 698 DSMQEMVGGLLQICFYTFVNKSLSVEFPEMLAEIISNQLPKFKDGSVKPLLFH 750